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Fig. 2 | Particle and Fibre Toxicology

Fig. 2

From: Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities

Fig. 2

Acute PM2.5 exposure impairs TJs of RPE in mice. A Expression of RPE65 and TJ protein Occludin in mouse RPE/choroidal tissues suggested by immunoblotting. GAPDH served as the internal control. PBS, phosphate buffer saline; TJ, tight junction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. B Quantification of shown blot, normalized by GAPDH (n = 3). C Immunofluorescence staining of TJ marker ZO1 on RPE flat-mounts in mice treated with PBS or PM2.5 for 3 days. Data in (B) are presented as mean ± SD. *p < 0.05

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