Fig. 8
Effect of PM2.5, the DRP1 inhibitor Mdivi-1 and the OPA1 activator BGP-15 on mitochondrial fission and fusion proteins and necroptosis-related proteins in lungs in mice. Effect of PM2.5, Mdivi-1 and BGP-15 on Western blot analysis of the mitochondrial fusion and fission proteins DRP-1 (A), phosphorylated DRP-1 (p-DRP1) (B) and OPA1 (C) after exposure to PM2.5. Effect of PM2.5 and Mdivi-1/BGP-15 on necroptosis related proteins expression in mice. Western blot analysis of the necroptosis related proteins mixed lineage kinase domain-like (MLKL) (D) and receptor-interacting protein kinase 1 (RIPK1) (E) after exposure to PM2.5 or pretreatment with Mdivi-1 or BGP-15. Mean values and data from individual experiments are shown. One-way ANOVA with Bonferroni’s post hoc test (for equal variance) or Dunnett’s T3 post-hoc test (for unequal variance) was performed for comparisons among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001