Fig. 6From: Attenuation of PM2.5-induced alveolar epithelial cells and lung injury through regulation of mitochondrial fission and fusionEffect of PM2.5, DRP1 knockdown (KD) and OPA1 overexpression (OE) on mitochondrial fission and fusion proteins expression, mitophagy- and necroptosis-related proteins in A549 cells. Western blot analysis of the mitochondrial fusion and fission proteins DRP1 (A), phosphorylated DRP1 (p-DRP1) (B), mitochondrial fission factor (MFF) (C), mitofusin2 (MFN2) (D) and OPA1 (E) after exposure to PM2.5 in DRP1-KD and OPA1-OE cells. Effect of PM2.5, DRP1-KD and OPA1-OE on the expression of the mitophagy-related proteins PTEN induced putative kinase 1 (PINK1) (F), sequestosome-1 (SQSTM1/p62) (G), recombinant Parkinson disease protein 2 (PARK2) (H) and microtubule-associated protein 1 A/1B-light chain 3 (LC3B) (I). Effect of PM2.5, DRP1-KD and OPA1-OE on the expression of the necroptosis-related proteins mixed lineage kinase domain-like (MLKL)(J), receptor-interacting protein kinase 1 (RIPK1) (K) and receptor-interacting protein kinase 3(RIPK3) (L) Mean values and data from individual experiments are shown. One-way ANOVA with Bonferroni’s post hoc test (for equal variance) or Dunnett’s T3 post hoc test (for unequal variance) was performed for comparisons among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001Back to article page