Fig. 5
Effect of PM2.5, the DRP1 inhibitor Mdivi-1 and the OPA1 activator BGP-15 on mitochondrial fission and fusion proteins, mitophagy- and necroptosis-related proteins in A549 cells. Western blot analysis of the mitochondrial fusion and fission proteins DRP1 (A), phosphorylated DRP1 (p-DRP1) (B), mitochondrial fission factor (MFF) (C), mitofusin2 (MFN2) (D) and OPA1 (E) after exposure to PM2.5 or pretreatment with Mdivi-1/BGP-15. Effect of PM2.5, Mdivi-1 and BGP-15 on the expression of the mitophagy-related proteins PTEN induced putative kinase 1 (PINK1) (F), sequestosome-1 (SQSTM1/p62) (G), recombinant Parkinson disease protein 2 (PARK2) (H) and microtubule-associated protein 1 A/1B-light chain 3 (LC3B) (I). Effect of PM2.5, Mdivi-1 and BGP-15 on the expression of the necroptosis-related proteins mixed lineage kinase domain-like (MLKL)(J), receptor-interacting protein kinase 1 (RIPK1) (K) and receptor-interacting protein kinase 3(RIPK3) (L) Mean values and data from individual experiments are shown. One-way ANOVA with Bonferroni’s post hoc test (for equal variance) or Dunnett’s T3 post hoc test (for unequal variance) was performed for comparisons among multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001