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Fig. 8 | Particle and Fibre Toxicology

Fig. 8

From: MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts

Fig. 8

Cultured in the conditioned media from Nano-CuO-exposed co-culture of BEAS-2B and U937* cells caused activation of unexposed MRC-5 fibroblasts. a is the experimental protocol of the co-culture experiments. Co-culture of BEAS-2B and U937* cells with ratio of 1:1 or 9:1 (BEAS-2B:U937*) was treated with 1 µg/mL of Nano-CuO for 12 h and the conditioned media (CM) were collected. MRC-5 fibroblasts were then cultured in the conditioned media for 0, 12, 24, 48, and 72 h. Proteins from MRC-5 cells were isolated to detect the expression of α-SMA, Col1A1, and fibronectin. b and d were the results of a single Western blot experiment. c and e were the average expression levels of α-SMA, Col1A1, and fibronectin normalized to β-actin from three independent experiments. Data represent mean ± SE (n = 3). * Significant difference as compared to the control group, p < 0.05. Graphics in a were created with BioRender (https://BioRender.com)

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Particle and Fibre Toxicology

ISSN: 1743-8977

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