Fig. 1

No evidence of AgNP aggregation and minimal release of Ag⁺ were detected following storage of AgNPs. A Nanoparticle tracking analysis was used to determine the hydrodynamic diameter of AgNPs in PBS, 30 min after dilution in cell culture media, and 72 h after dilution in cell culture media. The inset to the right shows the size distribution of particulate material in the media itself. B The peak from the hydrodynamic diameter for AgNPs measured in (A) was identified and the median values of five measurements per condition ± standard deviation are shown. C The ζ-potential distribution of AgNPs dispersed PBS at pH 7.4 after 100X dilution in water is shown. D The UV/visible light absorbance spectrum of AgNPs (arbitrary units (a.u.) is shown. E The fraction of Ag⁺ relative to total silver mass in a freshly prepared dispersion of AgNPs or following storage for 7 days is show. Statistical analysis was performed by Student T-test. Significant differences are indicated (*p < 0.05); n.s. not significant