Fig. 6

Quantitative analysis for the expression of genes involved in cumulus expansion, metabolism and cumulus response to oocyte signals. A Quantitative RT-PCR data for Has2, Ptx3 and Cd44 in COCs at the beginning (T₀) and after 3.5 h of culture without nanoparticles (Ctrl) and in presence of 10 μg/ml of uZnO and SiO₂ZnO NPs, respectively. B Quantitative RT-PCR data for Pfkp and Grem1 in COCs at the beginning (T₀) and after 3.5 h of culture without nanoparticles (Ctrl) and in presence of 10 μg/ml of uZnO and SiO₂ZnO NPs, respectively. P ≤ 0.05 is indicated by *, P ≤ 0.001 is indicated by **. With the exception of Pfkp, T₀ is always significantly different from Ctrl with P ≤ 0.001, with the exception of T₀ vs Ctrl for Has2, where P ≤ 0.05