Fig. 5
Evaluation of GFAP immunostaining in the brain. A Quantification of GFAP (astrocyte) positive % area per field at 400x at the end of treatment (EoT) and recovery (Rec) in the frontal cortex and hippocampus. Data are expressed as means ± SD. Statistical significance: ** = p < 0.01 vs Ctr (Kruskal-Wallis test, followed by Dunn’s multiple comparison test); b = p < 0.01 vs Ctr (Mann-Whitney test) (n = 5). Ctr = control group; 1Nominal dose (actual delivered doses were 0.20 and 0.80 mg/kg bw). B Representative images of GFAP immunostained sections of hippocampus (stratum lacunosum-moleculare), scale bar = 50 μm