Fig. 4From: LiCoO2 particles used in Li-ion batteries induce primary mutagenicity in lung cells via their capacity to generate hydroxyl radicalsLCO particles induce DNA strand breaks and DNA oxidative lesions in lung epithelial cells in vitro. Rat lung epithelial cells (RLE, 15600 cells/cm2) were exposed to culture medium (control, CTL), WC-Co, LCO or LTO and cytotoxicity was assessed after 24 h by the WST-1 assay (a). Alkaline comet assay, with or without oxidative DNA lesion repair enzyme (hOGG1), was performed 24 h after exposure to particles (b). *P < 0.05, **P < 0.01 and ***P < 0.001 (t-test between alkaline and alkaline + hOGG1 conditions). Bars represent means ± SEM (N = 4 with n = 4 for the WST-1 assay, N = 4 for the alkaline comet assay performed without hOGG1 and N = 2 for the alkaline comet assay performed with hOGG1, n = 2). ND = not determinedBack to article page