Fig. 7

The ROS scavengers inhibited BV2 cell death caused by N-GQDs. The effect of a total antioxidant Trolox (a) and a mitochondria targeted ROS scavenger MitoTEMPO (b) on the cell viability in BV2 cells treated with 100 μg/mL N-GQDs for 24 h were measured by CCK8 assay; c Representative flow cytometer dot plot of apoptosis and necrosis in BV2 cells treated with 100 μg/mL N-GQDs for 24 h pretreated with/without Trolox and MitoTEMPO were identified by using Annexin V-FITC/PI; d Quantitative results of necrotic and apoptotic percentages from flow cytometer analysis. Data are expressed as the mean ± SE of three independent experiments, performed in triplicate. Statistical significance was determined by one-way ANOVA and Dunnett’s t test (*P < 0.05, **P < 0.01, ***P < 0.001 vs. the control group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the 100 μg/mL N-GQDs group)