Influence of wood species on toxicity of log-wood stove combustion aerosols: a parallel animal and air-liquid interface cell exposure study on spruce and pine smoke

Table 4 Number of cells and cytokines from C57BL/6 J mice (n = 6) in the BALF and serum

Parameter	Unit	BALF			Serum
Parameter	Unit	Untreated	Spruce	Pine	Untreated	Spruce	Pine
Total cell number	10⁴ cells mL^− 1	50 ± 5.2	42 ± 7.1	117.6 ± 48.8	n.a.	n.a.	n.a.
Viability	%	82.5 ± 3.6	89.3 ± 3.4	92 ± 2	n.a.	n.a.	n.a.
Macrophages	10⁴ cells mL⁻¹	49.4 ± 5.2	41.3 ± 7.1	117.1 ± 48.7	n.a.	n.a.	n.a.
Neutrophils	10⁴ cells mL⁻¹	0.05 ± 0	0.3 ± 0.01	0.04 ± 0	n.a.	n.a.	n.a.
Lymphocytes	10⁴ cells mL⁻¹	0.4 ± 0.2	0.4 ± 0.01	0.4 ± 0.2	n.a.	n.a.	n.a.
Total protein	μg mL⁻¹	94.3 ± 5.2	67.4 ± 2.4	85.3 ± 10.5	n.a.	n.a.	n.a.
IFN-γ	Norm. pg mL⁻¹	0.30 ± 0.06	0.45 ± 0.12	0.43 ± 0.17	1 ± 0.24	0.7 ± 0.07	3.2 ± 2.69
IL-10	Norm. pg mL^− 1	2.37 ± 0.29	0.97 ± 0.47	3.05 ± 0.51	18 ± 0.97	12 ± 1.16	14 ± 1.99
IL-12p70	Norm. pg mL^− 1	15.30 ± 9.64	20.05 ± 4.74	17.12 ± 5.60	15 ± 4.26	21 ± 6.16	21 ± 5.6
IL-1b	Norm. pg mL⁻¹	0.85 ± 0.18	1.39 ± 0.12	0.88 ± 0.14	2 ± 0.15	2 ± 0.1	2 ± 0.1
IL-2	Norm. pg mL^− 1	2.15 ± 0.46	3.53 ± 0.45	1.88 ± 0.50	2.4 ± 0.32	1.6 ± 0.21	1.7 ± 0.14
IL-4	Norm. pg mL^− 1	0.93 ± 0.33	2.03* ± 0.16	1.20* ± 0.20	0.4 ± 0.06	0.8 ± 0.32	0.4 ± 0.16
IL-5	Norm. pg mL⁻¹	1.68 ± 0.55	2.15* ± 0.36	0.86* ± 0.11	3.5 ± 1.48	2.2 ± 0.51	1.2 ± 0.12
IL-6	Norm. pg mL^− 1	8.22 ± 1.68	*16.44 ± 0.50**	7.87* ± 1.52	6.2 ± 0.8	5.1 ± 0.52	5.3 ± 0.42
TNFα	Norm. pg mL^− 1	4.30 ± 0.55	6.58 ± 0.42	5.03* ± 0.51	15 ± 0.96	11 ± 1.1	9.3 ± 0.94
KC	Norm. pg mL⁻¹	14.74 ± 2.10	*55.79 ± 7.55**	28.46* ± 4.21	64 ± 4.18	56 ± 3.12	59 ± 3.44

Values are presented as the mean value ± SEM. Bold-faced number indicates a statistically significant difference from untreated mice (p < 0.05), * indicates a statistically significant difference between CCA exposures. Norm. pg mL-1 indicates standardization to the corresponding total protein amount; n.a. indicates not applicable

ISSN: 1743-8977