Fig. 9
Effects of IL-1RA on ICAM-1 and E-Selectin gene expression after exposure to Si10 or Min-U-Sil in 3D tri-cultures. The figure shows gene-expression on the apical versus the basolateral side of the 3D tri-culture. Cells in the apical compartment were pre-treated for 1 h with and without interleukin-1 receptor antagonist (IL-IRA; 30 μg/ml), and exposed for 10 h with and without Si10 (24 μg/cm2) or Min-U-Sil (192 μg/ cm2.) in the presence of the antagonist. The gene expression was determined by qPCR and represent the mean +/− of 3 independent experiments. *Statistical significant difference from control, #statistical significant difference from exposure without IL-1RA, P < 0.05