Fig. 5
Time-dependent gene expression of HO-1 on the apical and basolateral side of 3D tri-cultures after Si10 or Min-U-Sil exposure. The figure shows gene expression after 3, 6, 10 and 20 h exposure to 12 and 24 μg/cm2 Si10 (a, b) and 96 and 192 μg/cm2 Min-U-Sil (c, d). Gene expression was determined by qPCR and is presented as fold increase compared to control. Data represent the mean +/− SEM of 3–5 independent experiments. *Statistical significant difference from control at the same time-point, P < 0.05