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Table 2 Effects of DE exposure on the catecholamine system of GD28 fetuses

From: Repeated gestational exposure to diesel engine exhaust affects the fetal olfactory system and alters olfactory-based behavior in rabbit offspring

 

Control

Exposed

Effects of DE exposure

Effects of Sex

Exposure × Sex

Male (n = 4)

Female (n = 4)

Male (n = 4)

Female (n = 4)

F (1,15)

p-value

F (1,15)

p-value

F (1,15)

p-value

DA (fmol/mg)

274.9 ± 27.59

276.1 ± 37.85

389.7 ± 45.00

341.9 ± 41.79

3.502

0.088

0.525

0.484

0.262

0.619

DOPAC (fmol/mg)

212.1 ± 56.13

212.1 ± 43.34

346.0 ± 122.38

460.3 ± 84.89

2.451

0.146

0.453

0.515

1.308

0.277

HVA (fmol/mg)

222.6 ± 32.11

280.4 ± 32.21

514.1 ± 71.17

412.0 ± 134.76

2.761

0.125

0.357

0.562

0.311

0.588

DOPAC/DA

0.789 ± 0.240

0.789 ± 0.147

0.857 ± 0.247

1.33 ± 0.104

0.738

0.409

1.644

0.226

0.612

0.115

HVA/DA

0.810 ± 0.084

1.066 ± 0.214

1.315 ± 0.068

1.158 ± 0.247

0.411

0.534

0.016

0.901

0.612

0.451

(DOPAC+HVA)/DA

1.599 ± 0.290

1.855 ± 0.303

2.173 ± 0.307

2.488 ± 0.348

0.854

0.375

0.879

0.369

0.557

0.471

NA (fmol/mg)

414.7 ± 14.90

488.8 ± 45.88

304.0 ± 54.32

437.1 ± 145.76

1.945

0.191

1.808

0.206

0.431

0.525

MHPG (fmol/mg)

126.9 ± 17.15

94.45 ± 24.17

135.0 ± 31.76

137.9 ± 78.42

0.613

0.450

0.099

0.759

0.071

0.794

MHPG/NA

0.304 ± 0.36

0.192 ± 0.039

0.469 ± 0.102

0.277 ± 0.091

6.466

0.027*

5.416

0.040*

1.241

0.289

  1. Basal rates of Dopamine (DA), 3,4-Dihydroxyphenylacetic acid (DOPAC), Homovanillic acid (HVA), Noradrenalin (NA), and 3-Methoxy-4-hydroxyphenylglycol (MHPG), along with the correspondent ratios. Results were expressed as fentomoles/milligram of fresh tissues (fmol/mg). Results are shown as mean ± SEM. *p < 0.05 represented a significant difference between each group. Neither NA, nor its main metabolite MHPG, was significantly changed when compared to controls. However, the MHPG/NA ratio in exposed fetuses was higher when compared to controls. DA levels were slightly higher in exposed fetuses, even if not significant. No significant difference existed between groups regarding the other variables of interest