In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls

Table 2 Parameters that are necessary to calculate the delivered dose for the silver nanoparticles AgPAA and AgPURE in cell culture medium containing either 10% fetal calf serum (FCS) or 1% insulin, transferrin and selenium (ITS), using the 3DSDD model

	AgPAA FCS	AgPURE FCS	AgPURE ITS
effective density [g/cm³]	1.143	1.552	1.698
Particle number [#]	10,000	10,000	10,000
temperature [°C]	37	37	37
medium density [g/cm³]	1.0037	1.0037	1.0030
medium viscosity [mPa s]	0.725	0.725	0.703
filling level of the dish [cm]	0.88	0.88	0.88
height of cell growth on side wall [cm]	0.54	0.54	0.54
simulation time [h]	168	168	168
diameter of primary particles [nm]	6.8	13.8	13.8

Effective density, medium density and viscosity, and height of cell growth on the side wall were determined experimentally. The diffusion coefficient or hydrodynamic size distribution are not given in the table, as it was used as the complete distribution measured by NTA after 24 h (see Fig. 5)

ISSN: 1743-8977