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Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls

Fig. 4

Verification of the 3DSDD model by comparison with the published models DG and volumetric centrifugation method (VCM)-ISDD for nanoparticle incubations of up to 3 days. (a to b) The DG model was used to calculate the delivered dose of TiO2 and SiO2 nanoparticles using parameters from DeLoid et al. [7]. (c to d) The VCM-modified ISDD model was used to calculate the delivered dose of CeO2 and gold nanoparticles using parameters from DeLoid et al. [6]. The parameters used for calculation are given in Table 1. The same parameters were used for calculating the delivered dose with the 3DSDD model. For proper comparison 3DSDD calculations were done for cell on the bottom only, like in the DG/VCM-ISDD. In addition, 3DSDD results were calculated for differentiated Caco-2 cells growing on bottom and wall of the cell culture dish; these data are also shown in the graphs to visualize the impact of vertically growing cells on the total delivered dose. The inserts show the effective nanoparticle doses calculated with the 3DSDD model for differentiated Caco-2 cells as a subdivision into both fractions, cells on the bottom and cells on the wall of the cell culture dish. Abbreviations: 3DSDD: 3D-sedimentation-diffusion-dosimetry model; VCM: volumetric centrifugation method used for determining the effective density of nanoparticle dispersions; ISDD: In vitro Sedimentation, Diffusion and Dosimetry model; DG: Distorted Grid model

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