Fig. 2

Impact of different parameters on the calculation of the delivered dose by the in silico dosimetry model introduced in this study (3D-sedimentation-diffusion-dosimetry, 3DSDD), applied to the cell growth of differentiated Caco-2 cells. Graphs show the fraction of particles that reach the differentiated Caco-2 cell monolayer on the bottom and wall of the cell culture vessel. The total fraction of particles reaching cells is also presented. In silico calculations were repeated three times and mean and standard deviation (too small to be visible in the graphs) were calculated. Results are given as percentage of the initial particle number. The following parameters were used for calculation: particle diameter 50 nm, effective density of nanoparticles 1.5 g/cm³, particle number 10000, temperature 37 °C, density of cell culture medium 1.0037 g/cm³, viscosity of cell culture medium 0.725 mPa s, height of cell growth on the cell culture dish wall 0.54 cm, surface area of cell culture dish bottom 0.34 cm² (96-well plate), geometrical shape of cell culture dish is a cylinder, height of medium level in well 0.57 cm (corresponds to about 200 μL in 96-well, 570 μL in 48-well, 2.2 mL in 12-well and 5.5 mL in 6-well) and incubation time 24 h. In each diagram one parameter was altered: (a) time, (b) particle number, (c) area of well bottom, (d) hydrodynamic diameter of particles, (e) effective density of particles, (f) medium height