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Fig. 5 | Particle and Fibre Toxicology

Fig. 5

From: Differential effects of diesel exhaust particles on T cell differentiation and autoimmune disease

Fig. 5

Organic fraction of diesel exhaust particles impact T cell differentiation in vitro. Naïve CD4+ T cells were isolated from WT (C57BL/6 J) or Ahr−/− mice. At time 0 cells were exposed to a dose-response (8 doses) of SRM1650b OF, SRM2975 OF, or solvent control and cultured for 3 days. a Representative flow plots of WT (left) and Ahr−/− (right) Th17 differentiation at 5.0 μg/mL OC, measured by percent IL-17 positive cells. b SRM1650b OF enhanced Th17 differentiation at 10 and 5 μg/mL OC compared to solvent control (top left) and SRM2975 OF enhanced Th17 differentiation at 5μg/mL OC (bottom left). In Ahr−/− cells, SRM1650b OF lost theTh17 differentiation effect (top right) and SRM2975 OF had no effect on Th17 differentiation (bottom right). c Representative flow plots of WT Treg differentiation at 0.00001 μg/mL OC measured by percent FOXP3 positive cells. d SRM1650b OF has no effect on Treg differentiation (top). SRM2975 OF suppresses Treg differentiation at 0.00001 μg/mL OC (bottom). Results are mean ± SEM of (WT Th17 SRM1650b OF n = 5), (Ahr−/− Th17 SRM1650b OF n = 4), (WT Th17 SRM2975 OF n = 6), (Ahr−/− Th17 SRM2975 OF n = 3), and (WT Treg SRM1650b OF and SRM2975 OF n = 4), Significant differences among groups (p < 0.05) are indicated by an asterisk. Abbreviations: AHR, aryl hydrocarbon receptor; SRM, standard reference materials; OF, organic fraction; OC, organic carbon

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