Fig. 4
From: Developmental basis for intestinal barrier against the toxicity of graphene oxide
Identification of downstream targets for intestinal PKC-3 in the regulation of GO toxicity and translocation. a Effects of intestine-specific RNAi knockdown of pkc-3 on expressions of par-1, par-5, lgl-1, lin-5, nlp-29, and sec-8 in GO exposed nematodes. b Effects of intestine-specific RNAi knockdown of lgl-1 or sec-8 on GO toxicity in inducing intestinal ROS production. c Effects of intestine-specific RNAi knockdown of sec-8 on distribution and translocation of GO/Rho B. The pharynx (*) and the intestine (**) were indicated by asterisks. Single arrowhead indicates the spermatheca, and double arrowhead indicates the tail. d Effects of intestine-specific RNAi knockdown of sec-8 on intestinal permeability as indicated by the signals of Nile Red. The right shows the comparison of fluorescence intensity of Nile Red signals in intestinal cells. e Genetic interaction between PKC-3 and SEC-8 in the regulation of toxicity and translocation of GO in inducing intestinal ROS production. f Genetic interaction between PKC-3 and SEC-8 in the regulation of toxicity and translocation of GO/Rho B. The pharynx (*) and the intestine (**) were indicated by asterisks. Single arrowhead indicates the spermatheca, and double arrowhead indicates the tail. Acute exposure was performed from L4-larvae for 24 h. GO or GO/Rho B exposure concentration was 10 mg/L. Bars represent means ± SD. **P < 0.01 vs VP303 (if not specially indicated)