Fig. 4

Exposure to MCP230 increases the expression of Cyp1a1 and Cyp1b1 in an uptake- and oxidative stress-dependent manner. A549 cells were exposed to MCP230 (50 μg/cm²) and simultaneously treated with an antioxidant (PBN; 10 mM) or an uptake blocker cocktail (UB) for 4 h. Cyp1a1 (a) and Cyp1b1 (b) expression relative to Hprt was determined by RT-qPCR. Data represent mean ± SEM from one of two independent experiments, performed in triplicate. ^ap < 0.05, compared to Media group. ^bp < 0.05, compared to MCP50 group. ^cp < 0.05, compared to MCP230 group. ^dp < 0.05, compared to MCP230+PBN group, one-way ANOVA with Tukey’s multiple comparisons test