Fig. 7

Olfactory Habituation test. In the olfactory habituation test, sniffing responses elicited by repeated presentations of non-social odors (almond and banana) or social odor (same sex urine) were analyzed in males (a) and females (b). For the initial presentation of same-sex urine (U1), FA-exposed female mice showed a significantly higher number of sniffing bouts, nearly twice the number of bouts seen in the DE-exposed females (# p < 0.05; Two-way repeated-measures ANOVA, with Bonferroni correction). Habituation response was measured as a decreasing number of sniffing responses with repeated presentations of the same odor. DE-exposed mice of both sexes were unable to habituate to repeated presentation of same-sex urine, a social odor, while FA-exposed control mice of both sexes showed significant habituation toward same-sex urine. (*p < 0.05; **p < 0.01; ***p < 0.001; One-way repeated-measures ANOVA, with Dunn’s multiple comparison test). Graph plotted as mean ± SEM; FA M n = 9, DE M n = 10, FA F n = 8, DE F n = 7