Fig. 6

Immunostaining of liver samples following 6 weeks exposure to PbO-NPs. a-c PCNA in liver tissue. Arrows indicate PCNA-positive cells in liver. In controls, PCNA positive cells are mainly sinusoid lining cells while after treatment (a), PCNA positive are hepatocytes (b, c – black arrows). The highest amount of positive cells is present in mononuclear infiltrates (islands of extramedullary hematopoiesis, c - white arrow). d-f TUNEL in liver tissue. Arrows indicate TUNEL-positive cells, mainly sinusoid lining cells as e.g. Kupffer cells. g-i Na-K ATPase in control samples. Membranes of hepatocytes show high positivity of enzymes, cords of hepatocytes are commonly arranged in lobules. j-l Na-K ATPase in treated samples. White arrows indicate regions in lobules without positivity. Hepatocytes are irregularly organized, different sized and often binucleated. Scale bar in all panels = 100 μm