Fig. 3From: Pro-inflammatory adjuvant properties of pigment-grade titanium dioxide particles are augmented by a genotype that potentiates interleukin 1β processingViability of LPS-primed BMDMs after chasing with TiO2 +/− peptidoglycan or MDP. BMDMs from WT (a) and Nod2 m/m (b) mice were pre-stimulated for 3 h with LPS (10 ng/mL). Then BMDMs were incubated for 3 h with the indicated concentrations of TiO2 particles suspended in TCM alone (TCM), or TCM + 10 μg/mL MDP (MDP), or TCM + 10 μg/mL peptidoglycan (PGN). Cells were stained with PI and F4/80 antibody for murine macrophages and analysed with flow cytometry, and viability was determined with PI exclusion. Percentages of PI− cells in relation to the total number of detected events are shown. Data represent mean ± SD from two independent experiments with three replicates each, n = 6Back to article page