Skip to main content
Fig. 4 | Particle and Fibre Toxicology

Fig. 4

From: Impaired Ciliogenesis in differentiating human bronchial epithelia exposed to non-Cytotoxic doses of multi-walled carbon Nanotubes

Fig. 4

a Expression of ciliogenesis marker gene FOXJ1 and mucin genes MUC5AC and MUC5B in ALI day 1 (“d1”) and ALI day 28 (“d28”) BECs, following a 24 h exposure to MWCNTs during submerged culture. Bars indicate standard deviation and treatments are expressed as μg/cm2. Day 1 expression of these markers was over 1000-fold less than at day 28 in control and MWCNT treatments. By day 28, no treatment-related changes in FOXJ1 expression were observed in cells from any of the 3 donors. b Expression of other genes related to mucociliary differentiation signaling was also unaffected by MWCNT treatment, both at ALI day 1 and day 28 following exposure. Retinoid signaling genes RARRES1 and RDH12 were unaltered by MWCNTs or NG treatments. Cornulin (CRNN) was downregulated after 28 days in ALI culture in all treatment groups. Data was compiled from 3 donors, with significance determined by ANOVA (p-value >0.05 following Bonferroni comparison)

Back to article page