Fig. 2
a Histological cross-sections of differentiated cultures, stained with H&E (left) and PAS (right) for counting ciliated and goblet cells respectively. Representative images from vehicle controls (top) and MWCNT 1 μg/cm2 (bottom) are shown here. Scale bars represent 10 μm. b Ciliated and goblet cell counts of stained cultures. Ciliated and goblet cells per 500 μm length of membrane were counted for each treatment and counts from all 3 donors were pooled. Vehicle control (CoV) and MWCNT 1 μg/cm2 treatments performed post-differentiation (at day 20) were included for comparison (labeled “Post”). A significant (*p < 0.05, **p < 0.01 ANOVA, Bonferroni post hoc) dose-dependent decrease in ciliated cells was observed by day 28, though this decrease was not seen in goblet cells. Data compiled from 3 donors, with 5 slide sections per donor. Bars represent mean ± standard deviation (SD)