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Fig. 6 | Particle and Fibre Toxicology

Fig. 6

From: Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairment

Fig. 6

Representative flow cytograms and corresponding fluorescence photomicrographs showing (a) % reduction in acridine orange positive cells (b) reduced Lysotracker red staining (c) reduction in pHrodo dextran green fluorescence (marked with yellow arrows). Scale bar - 50 μm. Per view 50 cells and 4 views per group were analyzed. (d) Immunoblotting and (e) respective densitometry analysis of cytosolic release of cathepsin D in GCNF exposed cells. GAPDH was used as a loading control. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant

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Particle and Fibre Toxicology

ISSN: 1743-8977

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