Fig. 4From: Impaired lysosomal activity mediated autophagic flux disruption by graphite carbon nanofibers induce apoptosis in human lung epithelial cells through oxidative stress and energetic impairmentRepresentative TEM photomicrographs (a) and corresponding fluorescence microscopic images (b) portraying the autophagosomes accumulation in GCNF (25 μg/ml) exposed cells. In TEM image, inset of the red marked area present detailed structure of autophagosomes whereas in fluorescence images yellow arrows marked the GFP-LC3 puncta. Scale Bar – 20 μm. Per view 5 cells and 10 views per group were analyzed. (c) Quantification of autophagosomes per cell in fluorescence images. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. (d –1, 2) Effect of GCNF on autophagic proteins in dose dependent and time dependent manner analyzed through western blot. GAPDH was served as a loading control. Densitometry analysis is represented by the digits given above the respective blots and also provided in Additional file 2: Figure S2a, b. Values are expressed as mean ± SE of three independent experiment. *p<0.05 was considered as statistical significant. (e) Monodansyl cadaverine (MDC) staining of GCNF exposed cell. Scale Bar - 50 μmBack to article page