Skip to main content

Table 3 In vitro results of ROS, IL-8 mRNA expression and TEER measurement after exposure to CBNP

From: Biological effects of carbon black nanoparticles are changed by surface coating with polycyclic aromatic hydrocarbons

 

A549 cells

16HBE14o- cells

Calu-3 cells

Relative DCF fluorescence

IL-8

Relative DCF fluorescence

IL-8

TEER (%)

IL-8

P90

10 μg/ml

1.2 ± 0.2

1.2 ± 0.4

1.5 ± 0.2**

1.4 ± 0.2

103 ± 7

1.0 ± 0.2

50 μg/ml

2.1 ± 0.5*

2.1 ± 0.8**

3.2 ± 1.1**

1.7 ± 0.9

109 ± 8

1.1 ± 0.0

P90-BaP

10 μg/ml

1.1 ± 0.1

0.7 ± 0.2

1.3 ± 0.1

0.7 ± 0.3

97 ± 8

1.9 ± 0.6**

50 μg/ml

1.2 ± 0.1

0.7 ± 0.2

2.1 ± 0.1**

1.9 ± 1.8

94 ± 9

3.0 ± 0.9**

P90-9NA

10 μg/ml

0.9 ± 0.1

0.8 ± 0.2

1.1 ± 0.1

1.1 ± 0.5

105 ± 10

1.1 ± 0.1

50 μg/ml

1.2 ± 0.2

0.8 ± 0.2

2.1 ± 0.3**

2.0 ± 0.9*

104 ± 9

1.2 ± 0.1

AS-PAH

10 μg/ml

2.3 ± 0.2**

0.8 ± 0.3

1.8 ± 0.2**

1.1 ± 0.4

91 ± 7*

1.1 ± 0.2

50 μg/ml

3.0 ± 0.4**

0.8 ± 0.3

1.9 ± 0.1**

1.4 ± 0.8

90 ± 6*

2.1 ± 1.2**

  1. Data show the n-fold change compared to medium controls of relative DCF fluorescence in A549 and 16HBE14o- cells, the n-fold change of relative IL-8 mRNA expression in A549, 16HBE14o- and Calu-3 cells, respectively, and the changes of TEER in Calu-3 cells after 24 h CBNP exposure
  2. Data are mean ± SD. n = 3–5, *p < 0.05 and **p < 0.01 CBNP compared to medium controls analyzed by two-sided Student’s t-test for unpaired values (ROS analysis and TEER results) or Mann Whitney U test (mRNA expression data)