Fig. 3
From: Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection
Schematic overview of the experimental setup in vivo: C57BL/6 mice were infected intranasally with 5 × 104 PFU of MHV-68 (①). At day 28 post infection, when virus latency is established, the mice were instilled with 50 μg in 50 μl volume of spherical carbon nanoparticles (CNP) or double-walled fiber shaped carbon nanoparticles (DWCNT) after non-surgical intubation (②). Tissue samples of the lung were harvested 24 h after instillation for analysis of the metabolome, transcriptome, histology and viral load (③)