Fig. 5
From: Endocytosed nanoparticles hold endosomes and stimulate binucleated cells formation
Interference with membrane vesicles distribution. a: 3D-reconstruction of control cell and treated cell, red spots of transferrin were present in control (a), red spots and green spots of Ps particles were present in treated cell (b). The red spots of transferrin accumulated at midbody in control, but were short in treated cell. b: Surface Plot of transferrin in control (a) and treated cell (b), the fluorescence of transferrin distributed and gathered at midbody and poles regions of control cell. But in treated cell, the transferrin dispersed in cytoplasm randomly. c. Transferrin located closely to the LVLS contained 30 nm Ps particles (green) in 3D space. A magnified image showed the close tethers of transferrin vesicles and LVLS. d: The returning time of transferrin from cytoplasm to cell surface in treated cell delayed obviously. The red fluorescent intensity reduction in cytoplasm of control cell was faster than that in treated cell. The t1/2 of control cells was 2.5 min and the t1/2 of treated cells was 4.9 min. The transferrin vesicles were tethered by LVLS contained 30 nm Ps particles