Skip to main content

Table 2 Characterization of aerosols and deposition of NM on cells

From: Air-liquid interface exposure to aerosols of poorly soluble nanomaterials induces different biological activation levels compared to exposure to suspensions

Nebulizer (PALAS, AGK 2000)

TiO2 NM105

TiO2 NM101

TiO2 NM100

CeO2 NM212

Suspension concentration (g/L)

1

5

10

1

5

10

1

5

10

1

5

10

Aerosol concentration (mg/m3) (Gravimetry)

7.9

52.5

105.7

7.1

35.1

75.3

10.6

55.4

101.8

10.6

56.7

113.5

Total aerosol volume concentration (um3/cm3) (SMPS + OPC)

18.6

83.3

133

11.8

66.4

114

11.7

55.3

118.4

10.8

45.8

101.3

Aerosol effective densitya (g/cm3)

0.42

0.63

0.79

0.90

0.83

0.89

0.60

0.63

0.64

0.98

1.24

1.12

Aerosol VMDb (nm)

874

963

997

683

750

1060

1240

1360

1320

597

727

842

Volume geometric standard deviation

2.56

2.15

2.01

1.91

1.83

2.23

2.52

2.31

2.23

2.52

2.17

2.25

Aerosol GMDb (nm)

196

234

249

61

74

85

289

319

317

135

190

210

Theoretical deposited massc (μg/cm2 in 3 h)

1.5

10.1

20.4

1.4

6.8

14.5

2.0

10.7

19.6

2.0

10.9

21.9

Deposited massd(μg/cm2 in 3 h) (n = 4-7) (ICP-MS)

0.06 ± 0.01

0.66 ± 0.12

2.68 ± 0.6

0.22 ± 0.03

1.51 ± 0.11

3.15 ± 0.44

0.11 ± 0.01

0.50 ± 0.12

2.85 ± 0.30

0.22 ± 0.02

1.54 ± 0.08

3.26 ± 0.68

Deposited masse(μg/cm2 in 3 h) (n = 3) (QCM)

0.11 ± 0.01

0.72 ± 0.06

1.07 ± 0.02

0.14 ± 0.01

1.08 ± 0.39

2.43 ± 0.21

0.44 ± 0.08

1.43 ± 0.53

3.21 ± 0.69

0.24 ± 0.05

1.37 ± 0.17

2.97 ± 0.64

Deposition efficiencyf (%) (ICP-MS)

4.1

6.5

13.2

15.8

22.4

21.7

5.2

4.7

14.5

10.7

14.1

14.9

Deposition efficiencyf (%)(QCM)

7.1

7.1

5.2

10.5

15.9

16.7

21.7

13.4

16.4

11.8

12.5

13.6

  1. aAerosol density = Aerosol concentration (gravimetry) / total aerosol volume concentration (SMPS + OPC)
  2. bGeometric Mean Diameter (GMD) and Volumetric Mean Diameter (VMD) were measured with a Scanning Mobility Particle Sizer (SMPS) and an Optic Counter (OPC)
  3. cTheoretical deposited mass calculated when assuming 100 % deposition on cells: theoretical deposited mass = mass concentration of aerosol/volume of aerosol passing through exposure chambers during exposure
  4. dDeposited mass measured by Inductively Coupled Plasma - Mass Spectrometry (ICP-MS)
  5. eDeposited mass measured using Quartz Cristal Microbalances (QCM)
  6. fDeposition efficiency = Deposited mass measured / theoretical deposited mass calculated