Fig. 3
From: Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDermâ„¢)
3D model structure, growth and differentiation across the nano-RSMN protocol: The EpiDermTM model is a structurally differentiated, multi-layered model (a) of the human epidermis created from primary human keratinocytes. From arrival (a) to harvest (d) untreated control tissues in absence of cyt B were grown and imaged daily. The stratum corneum barrier layer increased in thickness as dividing keratinocytes moved upwards and differentiated. (Note: the trans-well insert layer detached during image preparation, (a, d). Comparison of the untreated control tissue (d) to tissues exposed to cyt B and acetone via the topical (e) and medium (f) exposure routes at the harvest time-point showed no differences in tissue development/structure