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Table 5 Mean tissue viability of aSiO2-susp, quartz dust DQ12, talc, and three organic pigments determined in the EpiOcularâ„¢ eye irritation test (protocol variant 2 for solid test substances a)

From: Eye irritation testing of nanomaterials using the EpiOcularâ„¢ eye irritation test and the bovine corneal opacity and permeability assay

Test substance

aSiO2-susp

aSiO2-susp

Quartz dust DQ12

Pigment Red 57:1

Pigment Yellow 95

Pigment Black 32

Talc

No. of test run

1st test run

2nd test run b

Volume or mass applied

50 μL

50 μL

15 mg c

12 mg c

9 mg c

8 mg c

16 mg c

Viability of tissue #1 relative to NC

67

89

73

98

87

97

93

Viability of tissue #2 relative to NC

90

107

82

98

95

79.6

103

Mean relative tissue viability (and ITV%)

78 (23)

98 (18)

77 (10)

98 (0)

91 (8)

88 (18)

98 (10)

  1. The mean tissue viability of 2 tissues / test group is expressed relative to the corresponding negative control (NC) value further indicating (in brackets) the relative inter-tissue variability (ITV%)
  2. a Protocol variant 2 for solid test substances: 6 h exposure duration for solids; 25 min post-exposure immersion for solids
  3. b Repeat of test run due to high variability >20 %
  4. c Corresponding to 50 μL bulk volume