Eye irritation testing of nanomaterials using the EpiOcular™ eye irritation test and the bovine corneal opacity and permeability assay

Table 3 Mean tissue viability of 16 OECD representative nanomaterials (and the Ag NM dispersant) determined in the EpiOcular™ eye irritation test (protocol variant 1 ^a)

Test material	TiO₂ NM-100	TiO₂ NM-101	TiO₂ NM-102	TiO₂ NM-103	TiO₂ NM-104	TiO₂ NM-105	ZnO NM-110	ZnO NM-111	SiO₂ NM-200	SiO₂ NM-203	CeO₂ NM-211	CeO₂ NM-212	Ag NM-300 K	Ag dispersant NM-300 K DIS	MWCNT NM-400	MWCNT NM-401	MWCNT NM-402
Volume or mass applied	28 mg ^b	22 mg ^b	23 mg ^b	17 mg ^b	12 mg ^b	6 mg ^b	12 mg ^b	25 mg ^b	6 mg ^b	2.5 mg ^b	61 mg ^b	28 mg ^b	50 μL	50 μL	46 mg ^c	32 mg ^c	8 mg ^c
Viability of tissue #1 relative to NC	99	107	105	79	70	97	96	116	107	88	92	81	42	62	88	109	98
Viability of tissue #2 relative to NC	83	107	106	79	86	106	100	113	105	91	70	81	48	71	95	105	100
Mean relative tissue viability (and ITV%)	91 (16)	107 (1)	106 (1)	79 (0)	78 (16)	102 (9)	98 (5)	114 (3)	106 (2)	90 (3)	81 (22 ^d)	81 (0)	45 (6)	66 (9)	92 (7)	107 (4)	99 (2)

The mean tissue viability of 2 tissues / test group is expressed relative to the corresponding negative control (NC) value further indicating (in brackets) the relative inter-tissue variability (ITV%)
^a Protocol variant 1: 30 min and 90 min exposure duration for liquids and solids, respectively; 12 min post-exposure immersion for both liquids and solids
^b Corresponding to 50 μL bulk volume
^c Corresponding to 2×50 μL bulk volume
^d Since the inter-tissue variance of the two tissues was >20 %, the corresponding acceptance criterion was not met. However, since all other acceptance criteria were met and due to the unambiguous result recorded for the test substance, the test was considered valid despite this deviation and therefore was not repeated

ISSN: 1743-8977