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Fig. 3 | Particle and Fibre Toxicology

Fig. 3

From: Multi-walled carbon nanotube induces nitrative DNA damage in human lung epithelial cells via HMGB1-RAGE interaction and Toll-like receptor 9 activation

Fig. 3

DNA damage in CNT-L-treated cells. a Immunofluorescent images of CNT-L-induced 8-oxodG and 8-nitroG formation in A549 cells. A549 cells were treated with CNT-L at indicated concentrations for 8 h at 37 °C, and 8-oxodG and 8-nitroG formation was examined by double immunofluorescent technique as described in Methods. Hoechst, Hoechst 33258. Magnification, X200. b Relative staining intensity of 8-nitroG formed in CNT-L-treated A549 cells. Staining intensity per cell was analyzed by an ImageJ software. Relative staining intensity of the control was set at 1. Data represent means ± SD of 8 (control) or 4 (CNT-L) independent experiments. *p < 0.05 and ***p < 0.001, compared with the control. c 8-NitroG formation in CNT-L-treated HBEpC cells. HBEpC cells were treated with CNT-L at indicated concentrations for 4 h at 37 °C, and immunofluorescent technique was performed. Magnification, X100. d Relative staining intensity of 8-nitroG formed in CNT-L-treated HBEpC cells. Data were quantitatively analyzed as described in (b). Data represent means ± SD of 3-4 independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001, compared with the control. Statistical analysis was performed by ANOVA followed by Tukey’s test

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