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Table 2 Relationship of stereological test probes and 3D structural quantification of lung pathologies in 2D microscopic images

From: Assessing particle and fiber toxicology in the respiratory system: the stereology toolbox

Parameter in 3D

Parameter 2D in section

Test probe

Counting event

Density

Final measurements

Volume

Area

Test point

Point (P) in test volume

VV = ∑P / total number of test points

Vtot = VV x V(ref)

Surface area

Boundary line

Test line

Line intersection (I) with surface area

SV = 2x∑I / total length of test lines

Stot = SV x V(ref)

Length

Transect

Test plane

Transect (Q) with test plane

LV = 2x∑Q / total area of test planes

Ltot = LV x V(ref)

Number

-

Disector

Particle event (Q) in test volume

NV = ∑Q / total disector volume

Ntot = NV x V(ref)