Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure under different cell culture conditions

Table 2 Nanoparticle doses

ZnO-NPs	Assays	[μg/ml]		[μg/well]		[μg/cm²]
concentration		low_ZnO	high_ZnO	low_ZnO	high_ZnO	low_ZnO	high_ZnO
ALI^a	all					0.62	6.23
96-well submerged	Cytotoxicity & reporter assays	2.1	21.2	0.21	2.12	0.62	6.24
24-well submerged	qRT-PCR & ELISA	1.2	11.8	1.2	11.8	0.63	6.21
6-well submerged	CLSM		13.1		26.2		6.23

^aas determined by QCM. Particle concentrations for submerged experiments were calculated based on the complete settling of NPs within the investigated incubation times. Growth areas were 0.33 cm² for 96-well plates, 1.9 cm² for 24-well plates and 4.2 cm² for 6-well insert. Administered volumes were 0.1 ml, 1 ml and 2 ml, respectively

ISSN: 1743-8977