Fig. 8

mRNA expression of transporters Abcb1 (P-gp) and Abcg2 (BCRP) transporters in isolated brain microvasculature endothelial cells and BCRP activity assessment at the BBB after IV injection of 1 mg/kg TiO₂ NPs in rats. mRNA transcription profiles for Abcb1 (a; b) and Abcg2 (c; d) were performed by RT-qPCR in isolated rat brain microvasculature endothelial cells of control and treated animals. Each data point represents the mean ± SEM of n = 4 animals and RT-qPCR was performed in duplicate for each single preparation of brain microvasculature endothelial cells. Statistical comparison was performed by two tailed Mann-Whitney test, *P < 0.05. BCRP activity (e) was estimated by the ratio between prazosin concentrations in brain and plasma (partition coefficient or Kp). Each data point represents the mean ± SD of n = 4 rats. Statistical comparison was performed by two-way ANOVA, *P < 0.05