Figure 3
From: Diesel exhaust particle exposure in vitro impacts T lymphocyte phenotype and function
Loss of ΔΨm but preserved ATP content after exposure of T lymphocytes to DEP. (A) Flow cytometry analysis of ΔΨm after staining with JC-1 in untreated T lymphocytes (left panel), T lymphocytes treated with E4 (middle panel) or E5 (right panel) particles (30 μg/ml for 24 h for both compounds). The results obtained in a representative experiment are shown. The numbers in the boxed areas represent the percentages of cells with hyperpolarized mitochondria. The percentages of cells with depolarized mitochondria are shown below the dashed line. (B) Mean percentage (and SD) of lymphocytes with depolarized mitochondria obtained from independent experiments performed in cells from 15 healthy donors is also shown. *p < 0.05 versus untreated cells. (C) ATP content detected by chemiluminescent assay in untreated and E4-and E5-treated T lymphocytes (30 μg/ml for 24 h for both compounds). Data are expressed as mean ± SD and are obtained from independent experiments performed in T lymphocytes from 5 out 15 randomly selected healthy donors.