Nitric oxide and superoxide mediate diesel particle effects in cytokine-treated mice and murine lung epithelial cells — implications for susceptibility to traffic-related air pollution

Table 2 BAL fluid indices and lung glutathione in mice 24 h after nose-only air or DEP inhalation exposure

	Filtered Air	DEP
BAL fluid cells per lung (x10 ³ )	n = 4	n = 4
Total Cells	85.3 ± 7.4	111 ± 12.3
Macrophages	84.9 ± 7.3	108 ± 11.1
Neutrophils	0.1 ± 0.1	1.4 ± 0.40*
Lymphocytes	0.2 ± 0.1	1.7 ± 0.7*
BAL fluid biochemistries
LDH (U/mL)	18.8 ± 3.1	12.6 ± 1.8
Total Protein (μg/mL)	62.9 ± 5.6	49.6 ± 11.1
Albumin (μg/mL)	15.1 ± 0.9	10.9 ± 3.8
Lung glutathione (nmol/g tissue)
GSH	4012 ± 371	4860 ± 561
GSSG	356 ± 35.1	427 ± 39.8
GSH:GSSG ratios	11.7 ± 1.5	11.3 ± 0.4

DEP exposures were at 2 mg/m³ for 4 h/d × 2 d. Data are expressed as the mean ± SEM. Asterisk (*) indicates significantly different than air-exposed mice ( p < 0.05).

ISSN: 1743-8977