Figure 5

Real-time PCR on SOD1 and HO-1 in aerosol exposed cultures. White, open bars represent mean values after 4 h post – incubation, black bars indicate 24 h timepoints. Individual data values are expressed as points. No relevant induction was measured for SOD1. After 4 h, SOD 1 values were 1.5 (SD 0.3), 1.7 (SD 0.1), 1.3 (SD 0.1) and 1.5 (SD 0.1) for Gas – control, 22, 45 and 90 sec particle production. The corresponding data for 24 h were 1.3 (SD 0.1), 1.7 (SD 0.6), 1.6 (SD 0.8), 1.4 (SD0.6). In contrast, HO-1 RNA levels were elevated after 4 and 24 h.