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Figure 6 | Particle and Fibre Toxicology

Figure 6

From: Mechanism of cellular uptake of genotoxic silica nanoparticles

Figure 6

(a) Fluorescence intensity of carboxyfluorescein-loaded vesicles in 20 mM MOPS, 30 mM Na 2 SO 4 , pH 7.4 after the addition of 10 and 100 μg/ml of silica NPs and 0.1% (v/v) Triton X-100, at times indicated by the arrows. The vesicles are made up of Lipid mix 1 (POPC:DOPE:DOPS:cholesterol at 9:6:1:4 weight ratio) and loaded with 50 mM carbofluorescein. (b) Leakage of carboxyfluorescein from 400 nm vesicles after the addition of silica NPs under various conditions, as indicated (MOPS = 20 mM MOPS, 30 mM Na2SO4, pH 7.4; DMEM = Dulbecco’s Modified Eagle’s medium; Lipid Mix 1 = POPC:DOPS:cholesterol at15:1:4 weight ratio; Lipid Mix 2 = POPC:DOPE:DOPS:cholesterol at 9:6:1:4 weight ratio). Leakage (as a percentage) was determined assuming 0% leakage prior to addition of NPs and 100% leakage after the addition of 0.1% (v/v) Triton X-100 as shown in (a).

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