Figure 4

The cell viability and induction of IL8 production in C3A cells treated with the ENPRA NMs for 24 hr. Cells pre-treated with the antioxidant Trolox (100 μM, 1 hr) are shown in grey, while cells not pre-treated are shown in black. Values represent mean ± SEM (n = 3), significance indicated by * = p < 0.05 and ** = p < 0.005, when nanomaterial treatments are compared to the control. ∞ = p < 0.05 and ∞∞ p < 0.005 is representative of significant difference between values signifying absence and presence of Trolox pre-treatment at each given concentration. A) NM 101 cytotoxicity B) NM 101 IL8 secretion C) NM 110 cytotoxicity D) NM 110 IL8 secretion E) NM 111 cytotoxicity F) NM 111 IL8 secretion G) NM 300 cytotoxicity H) 300 IL8 secretion I) NM 400 cytotoxicity J) NM 400 IL8 secretion K) NM 402 cytotoxicity L) NM 402 IL8 secretion M) NRCWE 001 cytotoxicity N) NRCWE 001 IL8 secretion O) NRCWE 002 cytotoxicity P) NRCWE 002 IL8 secretion Q) NRCWE 003 cytotoxicity R) NRCWE 003 IL8 secretion S) NRCWE 004 cytotoxicity T) NRCWE 004 IL8 secretion.