Figure 1

Mitochondrial activity was measured via the MTS assay (A) and membrane integrity was determined by the LDH assay (B) for monocultures of H441 and ISO-HAS-1 on 96 well plates (conventional monoculture). Cells were incubated with aSNP (Ludox TM-40: light grey, NexSil20: dark grey) for 4 h in serum-free medium. aSNPs were then removed and cells were cultivated for further 20 h. The assays were conducted after both time points (4 h exposure and 4 h exposure with 20 h recovery). Data are depicted as percentage of the untreated control (A: MTS) or as percentage of the total LDH amount of the cells (B: LDH, lysis control). Results are shown as means ± S.D. (n = 6-9) of 2-3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 compared to the untreated control