Figure 4
From: Darkfield-Confocal Microscopy detection of nanoscale particle internalization by human lung cells
Image galleries of BEAS cells exposed to nanoparticles for 5 and 120 min. Shown are optical sections taken from a confocal z-stack of nuclear fluorescence with darkfield detection of NP. Results show time-dependent internalization of nanoparticle uptake by human bronchial epithelial cells imaged by Darkfield (DF)/Confocal (CLSM) microscopy. BEAS-2B cells were exposed to 27 nm TiO2 particles for 5 min (top gallery) or 120 min (bottom gallery), fixed and stained with DAPI. The nanoparticles (depicted as white) were located in slices above the blue nucleus at 5 min but were on the same sections as the nuclei at 120 min. Cells were excited using the 404 laser line while fluorescence was monitored using the blue channel. Scale bar represents 2 μm. Magnification 3000x with a 5X zoom.