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Figure 1 | Particle and Fibre Toxicology

Figure 1

From: Particulate matter air pollution causes oxidant-mediated increase in gut permeability in mice

Figure 1

Particulate matter induces dose-dependent ROS generation and cell death in colonic epithelial cells. (A) Caco-2 cells stably expressing an oxidant-sensitive GFP probe (mito-Ro-GFP, lentivirus) were treated with control vehicle (media) or different doses of PM. The percentage oxidation of the probe was measured using flow cytometry 4 hours later. The t-butyl hydroperoxide (t-BOOH) was used as a positive control. (B) Caco-2 cells were treated as described above and cell death (DNA fragmentation via enzyme-linked immunosorbent assay) was measured 24 hours later. (*P < 0.05 PM compared with PBS, n ≥ 4).

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