Figure 5
From: Pulmonary exposure to carbon black nanoparticles and vascular effects
Expression of VCAM-1 and ICAM-1 on the endothelium and the presence of 3-nitrotyrosine (3NT) in the vascular tissue of aorta from 11-13 weeks old apoE-/- mice exposed to carbon black by i.t. instillation. The antibodies used were HRP-streptavidin conjugated and visualized with DAB. On the left, the data are depicted as geometric means of ranked values representing the amount of staining assessed in 4 sections of aorta from each animal. The medians are depicted as horizontal lines (n = 10-20); each symbol represent the result from one animal. On the right, images are shown of aorta sections representative of the median in the control group (left) and the 2.7 mg/kg exposed group (right). Binding of antibodies can be seen as brown staining of the tissue.