Lung inflammation and genotoxicity following pulmonary exposure to nanoparticles in ApoE-/- mice

Table 1 mRNAs of Mip-2, Mcp-1 and Il-6 in lung tissue and cell distribution and protein in BAL fluid 3 and 24 h after instillation of carbon black in C57 and ApoE^-/- mice.

		C57	C57	ApoE ^-/-	ApoE ^-/-
		Control	CB 54 μg	Control	CB 54 μg
3 h
lung tissue	*Mip-2*	9.6 ± 2.5	20.4 ± 5.9	10.2 ± 3.2	108.1 ± 15.1***
	*Mcp-1*	12.1 ± 4.6	20.6 ± 5.4	10.1 ± 1.2	265.5 ± 163.9***
	*Il-6*	4.0 ± 1.9	5.8 ± 2.3	2.1 ± 0.4	31.4 ± 4.8***
BAL	Neutrophils% ^a	3.4 ± 2.4	4.5 ± 0.4	3.7 ± 1.2	13.8 ± 10.9
	Macrophages% ^a	94.5 ± 2.6	93.8 ± 0.4	93.9 ± 2.0	83.1 ± 10.7
	Total BAL cells	55732 ± 14617	73407 ± 9267	83262 ± 4819	49417 ± 7700
	Protein	133.3 ± 21.7	82.4 ± 6.0*	102.5 ± 5.2	139.3 ± 17.3**
24 h
lung tissue	*Mip-2*	7.8 ± 1.0	82.8 ± 24.8***	5.1 ± 0.5	134.8 ± 33.2***
	*Mcp-1*	39.1 ± 10.4	434.1 ± 145.8***	28.3 ± 2.7	1087.0 ± 310.6***
	*Il-6*	2.1 ± 0.6	20.3 ± 12.3*	1.1 ± 0.1	44.0 ± 13.0***
BAL	Neutrophils% ^a	5.2 ± 1.2	51.0 ± 12.6**	5.3 ± 1.6	75.8 ± 3.4***
	Macrophages% ^a	92.6 ± 2.3	47.9 ± 12.7**	93.6 ± 1.5	22.1 ± 3.7***
	Total BAL cells	49022 ± 3589	98857 ± 11618	65290 ± 5246	78596 ± 21414
	Protein	114.5 ± 13.7	124.7 ± 10.6	110.6 ± 5.7	182.4 ± 7.1***

Results are given as mean ± SEM. Expression of mRNA is normalized to 18S rRNA and is multiplied by 10⁷. The data were analyzed by full three-factor ANOVA tests with strain, exposure and time as categorical variables. There were no triple interactions between the type of strain, exposure and time. ^a:Due to uneven variance, we determined 95; 99 and 99.9% confidence interval for means. *, **, *** refer to statistical significance p < 0.05, p < 0.01 and p < 0.001, respectively. Interactions: Mip-2, Strain*Exposure p < 0.05; Mcp-1, Strain*time p < 0.05, Strain*Exposure p < 0.05; Il-6, Exposure*Time p < 0.05, Strain*Exposure p < 0.05; Macrophages%, Exposure*Time p < 0.001, Strain*Exposure p < 0.01; Protein, Exposure*Time p < 0.01, Strain*Exposure p < 0.001.

ISSN: 1743-8977