Figure 5From: Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epitheliaIn situ XAS analysis of intracellular Ti-rich regions. Micro-XRF image of a cross-section of Caco-2 cells exposed for 24 h, on their apical pole, to 50 μg/mL of TiO2-NPs (A). Ap.: apical pole; bl.: basolateral pole. Phosphorous (P) distribution map is depicted in green and titanium (Ti) distribution map is depicted in red. The area pointed out with an arrow was further analysed by XAS. XAS spectra of reference Ti-acetate and TiO2 anatase nanopowders (5, 12 and 25 nm) and of Ti-rich regions in Caco-2 cells exposed for 12 h (Cells 12 h) or 24 h (Cells 24 h) to 50 μg/mL of 12 nm-diameter anatase TiO2-NPs (B). Focus on the pre-edge region (4972-4985 eV) and its deconvolution using an arctangent function and 4 Gaussian peaks (A1, A2, A3, B) (C). Solid line: recorded data, dashed line: fit. Panels indicate A2/A3, which is the ration of intensity of A2 to intensity of A3.Back to article page