Figure 6
Induction of micronuclei in A549 cells by CuO NP, CuO MP or CuCl 2 . Logarithmically growing A549 cells were incubated with CuO NP, CuO MP or CuCl2 for 24 h. The frequencies of micronuclei were determined according to [26] as described in Materials and methods. The data represent mean values of at least 3 independent experiments + SD. 1 μg/mL are equal to 0.2 μg/cm2 and 50 μg/mL CuO are equal to 630 μM Cu2+ in case of complete dissolution. n.d.: not determined.